DNA

Part:BBa_K4864000:Design

Designed by: Divakar K   Group: iGEM23_SVCE-CHENNAI   (2023-09-10)


Nitroreductase_NR3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 534
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is compactable with a bacterial expression system. Codon optimization is not required if expressed in E. Coli and B. megaterium strains.


Source

Metagenomic origin

References

Sree, B.R., Sowjanya, B. and Divakar, K., 2019. Metagenomic bioprospecting of novel oxygen insensitive nitroreductase for degradation of nitro aromatic compounds. International Biodeterioration & Biodegradation, 143, p.104737.

Bioproject ID: PRJNA345535